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KMID : 0861020010160020019
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Korea Journal of Herbology
2001 Volume.16 No. 2 p.19 ~ p.27
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Comparative studies on the angiogenic activity of water extract of Angelica Gigantis Radix , Angelica Sinensis Radix and Angelica Radix
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Jang Jun-Bock
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Abstract
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A rat sponge implant model was used to examine the angiogenic effect of water extract of Angelica Gigantis Radix (EAG). Angelica Sinensis Radix (EAS) and Angelica Radix (EAA). It was based on subcutaneous implantation of sterile polyester sponges in the Sprague Dawley (SD) rat and we have characterized a dye method that incorporates 5% carmine red in 10% gelatin. Vascularity index (V.I.) expressed as ㎍ dye/㎎ dry tissue. The result of study is as follows;
1. Subcutaneous implantation of sterile polyester sponges in SD rats enhanced the angiogenesis over 14 days, as determined by measurements of relative angiogenic effects by a vascularity index.
2. Daily oral administration (100 ㎎/㎏ for 14 days) of EAS and EAA produced weak effect on sponge-induced angiogenesis but EAG caused intense neovasculalization.
3. PBS (50 μ1) and EAG (100 ㎎/㎏) - induced angiogenesis represents a special form of angiogenesis.
4. The vasoconstrictor peptidem, angiotensin Ⅱ(AⅡ), in daily doses of 100 nmol, caused an intense neovascularization and coadministration of the AⅡ (100 nmol) and EAG (100 ㎎/㎏), AⅡ and EAS (100 ㎎/㎏) and AⅡ and EAA (100 ㎎/㎏) produced angiogenic effect. Especially, AⅡ (100 nmol) and EAG (100 ㎎/㎏) caused very potent angiogenesis.
We have shown that oral administration of EAG (100 ㎎/㎏) induced angiogenesis. Thus the sponge model offers a new means for continuous and reproducible studies on the controlling mechanisms of angiogenesis.
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KEYWORD
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